Welcome to LevSeqDash Documentation!

LevSeq-Dash is a user-friendly web application that provides a rich and interactive visualization dashboard for directed evolution experiments. It allows researchers to explore protein structures in 3D, analyze sequence variants, and make data-driven decisions about which mutations lead to enhanced activity.

🌐 Live Application: https://enzengdb.org/

📦 Source Code: GitHub Repository

Key Features

• 3D Protein Structure Visualization: Interactive protein viewer with Molstar integration

• Sequence-Function Database: Comprehensive storage of directed evolution experiment data

• Variant Analysis: Compare and analyze mutations across experiments

• Sequence Alignment: BLASTP-style pairwise alignment for finding related sequences

• Interactive Visualizations: Heatmaps, rank plots, and single-site mutagenesis plots

• Lab Data Management: Upload, manage, and analyze your own experiment data

Quick Start

Get started quickly with the Public Playground mode:

# Clone the repository
git clone https://github.com/ssec-jhu/levseq-dash.git
cd levseq-dash

# Build and run with Docker
docker build . -t levseq-dash:playground --no-cache
docker run -p 8050:8050 levseq-dash:playground

# Access at http://0.0.0.0:8050

For detailed setup instructions, see Usage Guide.

Note

This project is under active development. Features and APIs may change.

User Documentation

• Usage Guide
  • Installation
  • Configuration
  • Uploading Experiments
  • Exploring The Database
  • Single Experiment Page
  • Sequence Alignment

Developer Documentation

• Developer Guide
  • Getting Started
  • Architecture Overview
  • Data Flow
  • Configuration File and Settings
  • Adding New Features
  • Extending Data Manager
  • Testing
  • Debugging